Distribution of cylA, esp, asa1, hyl and gelE virulence genes among clinical isolates of Enterococcus faecium and Entrococcus faecalis

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Distribution of cylA, esp, asa1, hyl and gelE virulence genes among clinical isolates of Enterococcus faecium and Entrococcus faecalis

Background Enterococci are opportunistic pathogens causing severe urinary tract infections, surgical wound infections, bacteremia and bacterial endocarditis. Aggregation substance (asa1), hemolysin/ cytolysin (cylA), gelatinase (gelE), enterococcal surface protein (esp), hyaluronidase (hyl) and biofilm formation are the major virulence determinants responsible for pathogenicity of enterococci. ...

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Virulence Genes among Enterococcus faecalis and Enterococcus faecium Isolated from Coastal Beaches and Human and Nonhuman Sources in Southern California and Puerto Rico

Most Enterococcus faecalis and E. faecium are harmless to humans; however, strains harboring virulence genes, including esp, gelE, cylA, asa1, and hyl, have been associated with human infections. E. faecalis and E. faecium are present in beach waters worldwide, yet little is known about their virulence potential. Here, multiplex PCR was used to compare the distribution of virulence genes among ...

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Comparison of Virulence Gene Profiles of Enterococcus faecium and Enterococcus faecalis Chicken Neck Skin and Faeces Isolates

The objective of this study was to find out the distribution of major virulence determinants asa1, gelE, cylA, esp, and hyl by multiplex PCR in 132 Enterococcus faecium and 67 Enterococcus faecalis isolates originated from chicken neck skin samples at slaughterhouse and faeces samples from intensive broiler enterprises and rural poultry establishments. In the study, 31.2% (62/199) of the entero...

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Wide Distribution of Virulence Genes among Enterococcus faecium and Enterococcus faecalis Clinical Isolates

Enterococcus, a Gram-positive facultative anaerobic cocci belonging to the lactic acid bacteria of the phylum Firmicutes, is known to be able to resist a wide range of hostile conditions such as different pH levels, high concentration of NaCl (6.5%), and the extended temperatures between 5(°)C and 65(°)C. Despite being the third most common nosocomial pathogen, our understanding on its virulenc...

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Development of a multiplex PCR for the detection of asa1, gelE, cylA, esp, and hyl genes in enterococci and survey for virulence determinants among European hospital isolates of Enterococcus faecium.

A multiplex PCR for the simultaneous detection of five virulence genes (asa1, gelE, cylA, esp, and hyl) in enterococci was developed. The presence of these genes was investigated in 153 clinical and 118 fecal Enterococcus faecium isolates from inpatients at an increased risk of developing infections (such as patients in intensive care units and hematology wards) from 13 hospitals in eight Europ...

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ژورنال

عنوان ژورنال: BMC Infectious Diseases

سال: 2014

ISSN: 1471-2334

DOI: 10.1186/1471-2334-14-s3-p32